By D. Perlman (Ed.)

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In general the G C method agrees with taxonomic assignments based on other criteria and appears to have significance in yeast taxonomy. The G+C base ratio method, which only clearly demonstrates differences in nucleic acid structures, is complemented by the molecular hybridization method. , 1961), are related to an extent in their base sequences. ] In their studies on yeast Bak arid Stenderup (1969) obtained cell DNA and radioactive RNA, prepared by the action of a synthetase using DNA as a template.

G . , Brady, L. , and Robbers, J. E. 1. Pharni. Sci. 55,590-593. Walters, M . B. (1965). Mycologia 57,837-838. Wading, R. (1969). I n “Methods in Microbiology” (C. ), Vol. 4, Chemical Tests in Agaricology. in press. Academic Press, New York. Weir, J. , and Tyler, V. , Jr. ( 1 9 6 3 ) ~Pharrn. Sci. 52,419-422. This Page Intentionally Left Blank Proton Magnetic Resonance SpectroscopyAn Aid in Identification a n d C hemotaxonomy of Yeasts’ P. A. J. GORINAND J. F. T. SPENCER National Research Council of Canada, Prairie Regional Laborato y, Saskatoon, Saskatchewan, Canada I.

Also, many species of this genus live in association with bark beetles and their host trees, as d o yeasts of the genera Hansenula and Pichia. Some Cerutocystis species form hat- and Saturn-shaped ascospores, as do most species of the above yeasts. Most of the Ceratocystis species, unlike some of the other filamentous fungi, form mannose-containing polysaccharides. The genus Ceratocystis is of economic importance since it includes the pathogen Ceratocystis ulmi,the causal agent of Dutch Elm disease, and a number of other wilt pathogens and blue-stain fungi affecting the quality of lumber.

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